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Western blot analysis of KPNA6 in EL4 cell lysate with KPNA5 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry of KPNA5 in EL4 cells with KPNA5 antibody at 5 μg/mL.
Immunofluorescence of KPNA5 in EL4 cells with KPNA5 antibody at 20 μg/mL.
Western blot analysis of KPNA6 in EL4 cell lysate with KPNA5 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

KPNA5 Antibody

CATALOG NUMBER: 6655

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (94%)
Immunogen:
KPNA5 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human KPNA5.
The immunogen is located within amino acids 50 - 100 of KPNA5.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
KPNA5 antibody can be used for detection of EPAC1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
KPNA5 antibody is predicted to not cross-react with other Importin alpha family members.
Positive Control 1:
Cat. No. 1287 - EL4 Cell Lysate
Positive Control 2:
Cat. No. 17-207 - EL4 Cell Slide
Purification:
KPNA5 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
KPNA5 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
KPNA5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
KPNA5
Additional Names:
KPNA5 Antibody: SRP6, IPOA6, Importin subunit alpha-6, Karyopherin subunit alpha-5
Protein Accession Number:
CAH71948
Protein Gi Number:
55664992
Ncbi Gene Id Number:
3841
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
KPNA5 Antibody: Karyopherin, a cytosolic and heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC. KPNA5 belongs to a subfamily within the KPNA family that also includes KPNA4 and 6 and is thought to be involved in NLS-dependent protein imort into the nucleus.
Background Reference 1:
Moroianu J. Molecular mechanisms of nuclear protein transport. Crit. Rev. Eukaryot. Gene. Expr. 1997; 7:61-72.
Background Reference 2:
Gilchrist D and Rexach M. Molecular basis for the rapid dissociation of nuclear localization signals from karyopherin alpha in the nucleoplasm. J. Biol. Chem. 2003; 278: 51937-49
Background Reference 3:
Kohler MC, Fiebeler M, Hartwig S, et al. Evidence for distinct substrate specificity of importin alpha family members in nuclear protein import. Mol. Cell. Biol. 1999; 19:7782-91.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6655

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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