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Western blot analysis of extracts of various cell lines, using KRAS antibody (14-182) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of C6 cells using KRAS antibody (14-182) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using KRAS antibody (14-182) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using KRAS antibody (14-182) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using KRAS antibody (14-182) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

KRAS Antibody

CATALOG NUMBER: 14-182

Clonality:
Polyclonal
Tested Applications:
IF, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein of human KRAS
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, IP, WB
Application Note:
WB: 1:500 - 1:1000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:50 - 1:200
Positive Control 1:
A-549
Positive Control 2:
HeLa
Positive Control 3:
Raji
Positive Control 4:
Mouse spleen
Positive Control 5:
Mouse lung
Positive Control 6:
Rat brain
Predicted Molecular Weight:
Observed: 22kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
KRAS
Additional Names:
KRAS, C-K-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS3, RASK2, NS, CFC2
Ncbi Gene Id Number:
3845
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-182

List Size:
100 uL

List Price:

$436.00

Datasheet
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