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Antibody used in WB on Human Heart at 0.2-1 ug/ml.
Antibody used in WB on candida albicans Ras1P at: 1:5000.
Antibody used in WB on Human Heart at 0.2-1 ug/ml.

KRAS Antibody

CATALOG NUMBER: 27-016

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Drosophila, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Drosophila, Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human KRAS.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
KRAS antibody can be used for detection of KRAS by ELISA at 1:62500. KRAS antibody can be used for detection of KRAS by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. XBL-10407 - Fetal Heart Tissue Lysate
Predicted Molecular Weight:
22 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store KRAS antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
KRAS
Additional Names:
KRAS, C-K-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS3, RASK2, NS, CFC2
Protein Accession Number:
NP_203524
Protein Gi Number:
15718763
Ncbi Gene Id Number:
3845
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
KRAS is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma.This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region.
Background Reference 1:
Turbiner, J., (er) Mod. Pathol. (2008) In press.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-016

List Size:
100 ul

List Price:

$519.00

Datasheet
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