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Western blot analysis of KRT10 antibody in CEM cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human skin reacted with KRT10 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of KRT10 antibody in CEM cell line lysates (35ug/lane)

KRT10 Antibody

CATALOG NUMBER: 62-412

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This KRT10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 318-346 amino acids from the Central region of human KRT10.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
59 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
KRT10
Additional Names:
Keratin, type I cytoskeletal 10, Cytokeratin-10, CK-10, Keratin-10, K10, KRT10, KPP
Protein Accession Number:
P13645
Protein Gi Number:
269849769
Ncbi Gene Id Number:
3858
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
KRT10 is a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in its gene are associated with epidermolytic hyperkeratosis.
Background Reference 1:
Morais,P., EurDermatol 19 (4), 333-336 (2009)
Background Reference 2:
Barcelos,A.C., J. Cutan. Pathol. 36 (6), 647-654 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-412

List Size:
400 ul

List Price:

$495.00

Datasheet
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