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Western blot analysis of extracts of various cell lines, using KRT14 antibody (15-857) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded Rat lung using KRT14 antibody (15-857) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human esophagus using KRT14 antibody (15-857) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse kidney using KRT14 antibody (15-857) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of Rat skin using KRT14 antibody (15-857) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of Human skin using KRT14 antibody (15-857) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of Mouse skin using KRT14 antibody (15-857) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using KRT14 antibody (15-857) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

KRT14 Antibody

CATALOG NUMBER: 15-857

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human KRT14 (NP_000517.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:50 - 1:100
Positive Control 1:
mouse brain
Positive Control 2:
mouse heart
Positive Control 3:
mouse liver
Predicted Molecular Weight:
Observed: 56kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
KRT14
Additional Names:
KRT14, K14, NFJ, CK14, EBS3, EBS4
Ncbi Gene Id Number:
3861
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

15-857

List Size:
100 uL

List Price:

$436.00

Datasheet
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