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Antibody used in WB on Human HepG2 at 1.25 ug/ml.
Antibody used in IHC on Human Lung at 4.0-8.0 ug/ml.
Antibody used in WB on Human HepG2 at 1.25 ug/ml.

KRT18 Antibody

CATALOG NUMBER: 29-212

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human KRT18.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
KRT18 antibody can be used for detection of KRT18 by ELISA at 1:62500. KRT18 antibody can be used for detection of KRT18 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
47 kDa, 48 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store KRT18 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
KRT18
Additional Names:
KRT18, K18, CYK18
Protein Accession Number:
NP_000215
Protein Gi Number:
4557888
Ncbi Gene Id Number:
3875
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
KRT18 (Keratin 18) is type I intermediate filament chain. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis.KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.
Background Reference 1:
Ku, N.O., (2005) Gastroenterology 129 (3), 885-893.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

29-212

List Size:
100 ul

List Price:

$473.00

Datasheet
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