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Western blot analysis of extracts of MCF-7 cells, using KRT19 antibody (13-866).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded mouse liver using KRT19 antibody (13-866) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human mammary cancer using KRT19 antibody (13-866) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using KRT19 antibody (13-866) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using KRT19 antibody (13-866) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of Ags cells using KRT19 antibody (13-866).
Western blot analysis of extracts of MCF-7 cells, using KRT19 antibody (13-866).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

KRT19 Antibody [H2H10]

CATALOG NUMBER: 13-866

Clonality:
Monoclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant protein of human KRT19
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:5000 - 1:10000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
MCF7
Predicted Molecular Weight:
Observed: 44kDa
Purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
KRT19
Additional Names:
KRT19, CK19, K19, K1CS, MGC15366
Ncbi Gene Id Number:
3880
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-866

List Size:
50 uL

List Price:

$365.00

Out of stock

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