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Antibody used in WB on Human DLD1 at 1.25 ug/ml.
Antibody used in WB on Human DLD1 at 1.25 ug/ml.

KRT20 Antibody

CATALOG NUMBER: 29-769

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human KRT20.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
KRT20 antibody can be used for detection of KRT20 by ELISA at 1:312500. KRT20 antibody can be used for detection of KRT20 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
DLD1 Cell Lysate
Predicted Molecular Weight:
48 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store KRT20 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
KRT20
Additional Names:
KRT20, CD20, CK20, K20, KRT21, MGC35423, CK-20
Protein Accession Number:
NP_061883
Protein Gi Number:
27894337
Ncbi Gene Id Number:
54474
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
KRT20 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa.The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Background Reference 1:
Wong, C.S., (2008) Int. J. Surg. Pathol. 16 (2), 119-126.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

29-769

List Size:
100 ul

List Price:

$473.00

Datasheet
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