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Western blot analysis in K562 cell line lysates (35ug/lane).
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in K562 cell line lysates (35ug/lane).

KRT25 Antibody

CATALOG NUMBER: 55-737

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This KRT25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 380-408 amino acids from the C-terminal region of human KRT25.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
49 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
KRT25
Additional Names:
Keratin, type I cytoskeletal 25, Cytokeratin-25, CK-25, Keratin-25, K25, Keratin-25A, K25A, Type I inner root sheath-specific keratin-K25irs1, KRT25 (HGNC:30839)
Protein Accession Number:
Q7Z3Z0
Protein Gi Number:
74723316
Ncbi Gene Id Number:
147183
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the type I (acidic) keratinfamily, which belongs to the superfamily of intermediate filament(IF) proteins. Keratins are heteropolymeric structural proteinswhich form the intermediate filament. These filaments, along withactin microfilaments and microtubules, compose the cytoskeleton ofepithelial cells. The type I keratin genes are clustered in aregion of chromosome 17q12-q21.
Background Reference 1:
Rose, J. Phd, et al. Mol. Med. (2010) In press :
Background Reference 2:
Schweizer, J., et al. J. Cell Biol. 174(2):169-174(2006)
Background Reference 3:
Rogers, M.A., et al. Differentiation 72 (9-10), 527-540 (2004) :
Background Reference 4:
Hesse, M., et al. Eur. J. Cell Biol. 83(1):19-26(2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-737

List Size:
400 ul

List Price:

$495.00

Datasheet
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