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Antibody used in WB on Human Jurkat 0.2-1 ug/ml.
Antibody used in IHC on Human Intestine at 4.0-8.0 ug/ml.
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.

KRT2A Antibody

CATALOG NUMBER: 29-634

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human KRT2A.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
KRT2A antibody can be used for detection of KRT2A by ELISA at 1:312500. KRT2A antibody can be used for detection of KRT2A by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
70 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store KRT2A antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
KRT2
Additional Names:
KRT2A, KRT2E, KRTE, K2e, CK-2e, KRT2A
Protein Accession Number:
NP_000414
Protein Gi Number:
47132620
Ncbi Gene Id Number:
3849
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
KRT2A is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is expressed largely in the upper spinous layer of epidermal keratinocytes and mutations in this gene have been associated with bullous congenital ichthyosiform erythroderma.The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is expressed largely in the upper spinous layer of epidermal keratinocytes and mutations in this gene have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
Background Reference 1:
Bloor, B.K., (2003) Am. J. Pathol. 162 (3), 963-975.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

29-634

List Size:
100 ul

List Price:

$519.00

Datasheet
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