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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
KRT6B Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human cervical carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).

KRT6B Antibody

CATALOG NUMBER: 57-231

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This KRT6B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 207-236 amino acids from the Central region of human KRT6B.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
60 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
KRT6B
Additional Names:
Keratin, type II cytoskeletal 6B, Cytokeratin-6B, CK-6B, Keratin-6B, K6B, Type-II keratin Kb10, KRT6B, K6B, KRTL1
Protein Accession Number:
P04259
Protein Gi Number:
238054404
Ncbi Gene Id Number:
3854
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of thekeratin gene family. The type II cytokeratins consist of basic orneutral proteins which are arranged in pairs of heterotypic keratinchains coexpressed during differentiation of simple and stratifiedepithelial tissues. As many as six of this type II cytokeratin(KRT6) have been identified; the multiplicity of the genes isattributed to successive gene duplication events. The genes areexpressed with family members KRT16 and/or KRT17 in the filiformpapillae of the tongue, the stratified epithelial lining of oralmucosa and esophagus, the outer root sheath of hair follicles, andthe glandular epithelia. Mutations in these genes have beenassociated with pachyonychia congenita. The type II cytokeratinsare clustered in a region of chromosome 12q12-q13. [provided byRefSeq].
Background Reference 1:
Millar, E.K., et al. J. Clin. Oncol. 27(28):4701-4708(2009)
Background Reference 2:
Schweizer, J., et al. J. Cell Biol. 174(2):169-174(2006)
Background Reference 3:
Takahashi, K., et al. J. Biol. Chem. 270(31):18581-18592(1995)
Background Reference 4:
Rasmussen, H.H., et al. Electrophoresis 13(12):960-969(1992)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-231

List Size:
400 ul

List Price:

$495.00

Datasheet
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