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IHC testing of human colon carcinoma stained with KRT8 antibody (K8.8).
Western blot testing of HeLa cell lysate using KRT8 antibody (clone K8.8) at 1ug/ml. Observed molecualr weight: 46~50kDa.
IHC testing of human colon carcinoma stained with KRT8 antibody (K8.8).

KRT8 Antibody [K8.8]

CATALOG NUMBER: 33-166

Clonality:
Monoclonal
Tested Applications:
Flow, IF, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Human carcinoma keratin was used as the immunogen for this KRT8 antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC, WB
Application Note:
Western blot: 0.5-1 ug/ml
Flow Cytometry: 0.5-1 ug/million cells
IF: 1-2 ug/ml
IHC (FFPE): 0.5-1.0 ug/ml for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)
Differences in protocols and secondaries may require the KRT8 antibody to be titered for optimal performance.

1. FFPE staining requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Specificity:
Does not react with rat
Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
KRT8
Additional Names:
KRT8, CARD2, CK8, CYK8, K2C8, K8, KO, CK-8
Ncbi Gene Id Number:
3856
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
KRT8, also called Cytokeratin 8, is a low molecular weight keratin of approximately 50 kDa. It participates in cellular differentiation and signal transduction, protects against apoptosis, stress and injury, and helps maintain cellular structural integrity. KRT8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-166

List Size:
100 ug

List Price:

$626.00

Datasheet
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