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Western blot analysis using KSHV K8alpha monoclonal antibody against TPA induced BCBL - 1 cell lysate.
Immunochemistry staining of TPA - induced BCBL - 1 cells using monoclonal antibody to KSHV K8a. HRP - anti - mouse was used as the second antibody before color development with AEC.
Western blot analysis using KSHV K8alpha monoclonal antibody against TPA induced BCBL - 1 cell lysate.

KSHVK8a Antibody [8C12G10G1]

CATALOG NUMBER: 32-182

Clonality:
Monoclonal
Tested Applications:
ICC, WB
Host Species:
Mouse
Species Reactivity:
Virus
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Virus
Immunogen:
Ni-NTA purified recombinant human KSHV K8α expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ICC, WB
Application Note:
Western Blot:Dilution 1:500 - 1,000
ICC:Dilution 1:50 - 1:100
Determining optimal working dilutions by titration test.
Clonality:
Monoclonal
Isotype:
IgG2a
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
KSHV K8α monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
K8
Additional Names:
E3 SUMO-protein ligase K-bZIP
Protein Accession Number:
Q2HR82
Protein Gi Number:
123831254
Ncbi Gene Id Number:
4961462
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Kaposi's sarcoma-associated herpesvirus (KSHV) belongs to the gamma-(2)-herpesvirus subfamily and has been closely linked to the Kaposi’s sarcoma, primary effusion lymphoma (PEL) and multicentric Castleman’s disease. The genome of KSHV is 165-170 kb and contains at least 88 open reading frames. KSHV K8 and K8.1 open reading frames are juxtaposed and span from nucleotide (nt) 74850 to 76695 of the virus genome. A K8 pre-mRNA overlaps the entire K8.1 coding region, and alternative splicing of KSHV K8 and K8.1 pre-mRNAs each produces three isoforms (α,β, and γ) of the mRNAs.
Background Reference 1:
James J et al. Proc. Natl. Acad. Sci Vol. 1996. 93, pp. 14862-14867.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-182

List Size:
0.1 mL

List Price:

$495.00

Datasheet
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