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Western blot analysis using KSHV ORF45 monoclonal antibody against KSHV ORF45 recombinant protein
Immunocehmistry staining of TPA - induced BCBL - 1 cells using monoclonal antibody 2D4A5 clone to KSHV ORF45. HRP - anti - mouse was used as the second antibody before color development with DAB.
Western blot analysis using KSHV ORF45 monoclonal antibody against KSHV ORF45 recombinant protein

KSHVORF45 Antibody [2D4A5]

CATALOG NUMBER: 32-228

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Mouse
Species Reactivity:
Virus
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Virus
Immunogen:
Ni-NTA purified recombinant human KSHV ORF45 expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Western Blot:1:500 - 1,000
ELISA:Determining optimal working dilutions by titration test.
Clonality:
Monoclonal
Isotype:
IgG1
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
to KSHV ORF45 monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ORF45
Additional Names:
Protein ORF45
Protein Accession Number:
F5HDE4
Protein Gi Number:
550540839
Ncbi Gene Id Number:
4961474
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Kaposi's sarcoma-associated herpesvirus (KSHV) belongs to the gamma-(2)-herpesvirus subfamily and has been closely linked to the Kaposi’s sarcoma, primary effusion lymphoma (PEL) and multicentric Castleman’s disease. The genome of KSHV is 165-170 kb and contains at least 88 open reading frames. ORF45 protein encoded by an immediate-early gene in the KSHV genome is characterized as a phosphorylated protein, and it is localized in the cytoplasm of infected cells. Studies have shown that ORF45 Protein interacted with cellular IRF-7 and blocked virus-mediated phosphorylation and nuclear translocation of IRF-7. In consequence, ORF45 efficiently inhibited virus-induced production of type I IFN. Zhu et al (2003) reported that the ORF45 Protein was associated with purified virions.
Background Reference 1:
Chang Y. et al. 1994. Science. 266:1865-1869.
Background Reference 2:
Zhu FX. et al. 2002. PNAS. 99: 5573-5578.
Background Reference 3:
Zhu FX. et al. J Virol. 2003. April (77): 4221-4230.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-228

List Size:
0.1 mL

List Price:

$495.00

Datasheet
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