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Western blot analysis using KSHV ORF62 monoclonal antibody against KSHV ORF62 recombinant protein
Immunochemistry stating of TPA - induced BCBL - 1 cells using monoclonal antibody 5B7B6 clone to KSHV ORF62. HRP - anti - mouse was used as the second antibody before color development with AEC.
Western blot analysis using KSHV ORF62 monoclonal antibody against KSHV ORF62 recombinant protein

KSHVORF62 Antibody [5B7B6]

CATALOG NUMBER: 32-229

Clonality:
Monoclonal
Tested Applications:
WB
Host Species:
Mouse
Species Reactivity:
Virus
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Virus
Immunogen:
Ni-NTA purified recombinant human KSHV ORF62 expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot:1:500 - 1,000
ELISA:Determining optimal working dilutions by titration test.
Clonality:
Monoclonal
Isotype:
IgM
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
to KSHV ORF62 monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ORF62
Additional Names:
Triplex capsid protein VP19C homolog
Protein Accession Number:
F5H8Y5
Protein Gi Number:
550600169
Ncbi Gene Id Number:
4961461
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Kaposi's sarcoma-associated herpesvirus (KSHV) belongs to the gamma-(2)-herpesvirus subfamily and has been closely linked to the Kaposi’s sarcoma, primary effusion lymphoma (PEL) and multicentric Castleman’s disease. The genome of KSHV is 165-170 kb and contains at least 88 open reading frames. At least five major proteins are likely to be involved in the assembly of the HHV-8 capsid, including a protease (encoded by ORF17), the major capsid protein (encoded by ORF25), and three other smaller capsid proteins (encoded by ORF62, ORF26, and ORF65). Previous structural studies have shown that the HSV-1 triplex is a monomer of VP19c and a dimer of VP23 and that the HCMV triplex is similarly composed of a monomer and a dimer. By analogy, the KSHV triplexes are likely also composed of a monomer of the ORF62 protein and a dimer of the ORF26 protein, which are the respective homologs of VP19c and VP23.
Background Reference 1:
Chang Y. et al. 1994. Science. 266:1865-1869.
Background Reference 2:
Russo J.J. et al. 1996. PNAS. 93: 14862-14867.
Background Reference 3:
Wu L. et al. 2000. J VirolOct; 74(20):9646-54.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-229

List Size:
0.1 mg

List Price:

$408.00

Datasheet
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