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45-817 (0.1ug/ml) staining of HeLa cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing KU70 and probed with 45-817 (mock transfection in first lane).
45-817 (0.1ug/ml) staining of HeLa cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

KU70 Antibody

CATALOG NUMBER: 45-817

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: YKTEGDEEAEEEQ-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 70kDa band observed in lysates of cell lines A431, HeLa and HepG2 (calculated MW of 69.8kDa according to NP_001460.1). In transfected HEK293 transiently expressing KU70 a band of approx. 70kDa is observed. This band is not observed in the non-transfected HEK293.Recommended concentration: 0.1-0.3ug/ml.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
XRCC6
Additional Names:
XRCC6, KU70, X-ray repair complementing defective repair in Chinese hamster cells 6 (Ku autoantigen, 70kDa) , CTC75, CTCBF, G22P1, ML8, TLAA , ATP-dependent DNA helicase II, 70 kDa subunit, CTC box binding factor 75 kDa subunit, Ku autoantigen p70 subunit, OTTHUMP00000028581, thyroid autoantigen 70kD (Ku antigen), thyroid autoantigen 70kDa (Ku antigen), thyroid-lupus autoantigen p70
Protein Accession Number:
NP_001460.1
Protein Gi Number:
4503841
Ncbi Gene Id Number:
2547
Background Reference 1:
Ribes-Zamora A, Mihalek I, Lichtarge O, Bertuch AA. Distinct faces of the Ku heterodimer mediate DNA repair and telomeric functions. Nat Struct Mol Biol. 2007 Apr;14(4):301-307. Epub 2007 Mar 11.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-817

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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