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FACS testing of K562 cells: Black=cells alone; Green=isotype control; Red=Ku70 + Ku80 antibody PE conjugate
FACS testing of K562 cells: Black=cells alone; Green=isotype control; Red=Ku70 + Ku80 antibody PE conjugate

Ku70 + Ku80 Antibody [KU729]

CATALOG NUMBER: 33-138

Clonality:
Monoclonal
Tested Applications:
Flow, ICC, IF
Host Species:
Mouse
Species Reactivity:
Human, Primate
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Primate
Immunogen:
Nuclear extract of human HL-60 cells was used as the immunogen for this Ku70 + Ku80 antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, ICC, IF
Application Note:
Flow cytometry: 0.5-1ug/10e6 cells
IF: 0.5-1.0 ug/ml
Immunocytochemistry (Acetone-fixed cells): 0.25-0.5 ug/ml for 30 minutes at RT
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Ku70 + Ku80 antibody to be titered up or down for optimal performance.
Specificity:
Does not react with mouse, rat, chicken
Purification:
Protein G purified monoclonal antibody
Clonality:
Monoclonal
Isotype:
IgG1, kappa
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
XRCC6
Additional Names:
X-ray repair cross-complementing protein 6, 364-, 4299-, 5'-deoxyribose-5-phosphate lyase Ku70, 5'-dRP lyase Ku70, 70 kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, CTC box-binding factor 75 kDa subunit, CTC75, CTCBF, DNA repair protein XRCC6, Lupus Ku autoantigen protein p70, Ku70, Thyroid-lupus autoantigen, TLAA, X-ray repair complementing defective repair in Chinese hamster cells 6, XRCC6, G22P1
Ncbi Gene Id Number:
2547
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
This antibody recognizes a dimer of two proteins of 70kDa (Ku70) and ~80kDa (Ku80), identified as two subunits of Ku. Antbody KU729 recognizes a conformational epitope of the Ku70 + Ku80 dimer, which is destroyed during Western blotting. The Ku70 + Ku80 dimer is important for function of a 460kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-138

List Size:
100 ug

List Price:

$626.00

Datasheet
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