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SDS-PAGE Analysis of Purified, BSA-Free L1CAM Antibody (clone UJ127). Confirmation of Integrity and Purity of the Antibody.
SDS-PAGE Analysis of Purified, BSA-Free L1CAM Antibody (clone UJ127). Confirmation of Integrity and Purity of the Antibody.

L1CAM Antibody [UJ127]

CATALOG NUMBER: 33-172

Clonality:
Monoclonal
Tested Applications:
Flow, IF, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
A homogenous suspension of 16 week human fetal brain was used as the immunogen for this L1CAM antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, WB
Application Note:
Flow Cytometry: 0.5-1 ug/million cells
IF: 1-2 ug/ml
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1, kappa
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
L1CAM
Additional Names:
Neural cell adhesion molecule L1, N-CAM-L1, NCAM-L1, CD171, L1CAM, CAML1, MIC5
Ncbi Gene Id Number:
3897
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
This antibody recognizes a cell surface protein of 220-240kDa, identified as L1CAM (L1 cell adhesion molecule). The L1CAM gene, which is located in Xq28, is involved in three distinct conditions: 1) HSAS (hydrocephalus-stenosis of the aqueduct of Sylvius); 2) MASA (mental retardation, aphasia, shuffling gait, and adducted thumbs); and 3) SPG1 (spastic paraplegia). L1CAM also plays an important role in axon growth, fasciculation, and neural migration as well as in mediating neuronal differentiation. Expression of L1 protein is restricted to tissues arising from neuroectoderm. antibody to L1CAM is useful in the identification of primitive neuroectodermal tumors. The antibody binds to tumors of neuroectodermal and glial origin e.g. neuroblastoma and Schwannomas. It does not bind to pediatric or adult brain.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-172

List Size:
100 ug

List Price:

$626.00

Datasheet
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