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Western blot analysis of L1TD1 in Jurkat cell lysate with L1TD1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Western blot analysis of L1TD1 in Jurkat cell lysate with L1TD1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

L1TD1 Antibody

CATALOG NUMBER: 6439

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (76%)
Immunogen:
L1TD1 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human L1TD1.
The immunogen is located within amino acids 690 - 740 of L1TD1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
L1TD1 antibody can be used for detection of L1TD1 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Specificity:
L1TD1 antibody is predicted to not cross-react with other ECAT protein family members
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Purification:
L1TD1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
L1TD1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
L1TD1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
L1TD1
Additional Names:
L1TD1 Antibody: ECAT11, ECAT11, LINE-1 type transposase domain-containing protein 1, ES cell-associated protein 11
Protein Accession Number:
NP_061952
Protein Gi Number:
157694505
Ncbi Gene Id Number:
54596
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
L1TD1 Antibody: L1TD1 (ECAT11) is a member of the family of Embryonic Stem Cell Associated Transcripts (ECAT) that play an important role in stem cell biology. ECAT4 (Nanog) is a master regulator for the maintenance of mESC and hESC. ECAT5 was identified to be Embryonic stem cell expressed Ras (ERas), a Ras-like oncogene that is important in regulating mESC proliferation. ECAT9 (GDF3) is an important factor that helps maintain mESC pluripotency by inhibiting bone morphogenetic protein (BMP) signaling. L1TD1 is highly enriched in undifferentiated hESC and L1TD1 is a target gene of Nanog.
Background Reference 1:
Iwabuchi KA, Yamakawa T, Sato Y, et al. ECAT11/L1td1 is enriched in ESCs and rapidly activated during iPSC generation, but it is dispensable for the maintenance and induction of pluripotency. PLoS One. 2011; 6:e20461.
Background Reference 2:
Mitsui K, Tokuzawa Y, Itoh H, et al. The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. Cell 2003; 113:631-42
Background Reference 3:
Takahashi K, Mitsui K, Yamanaka S. Role of ERas in promoting tumour-like properties in mouse embryonic stem cells. Nature 2003; 423:541-5
Background Reference 4:
Wong RC, Ibrahim A, Fong H, et al. L1TD1 is a marker for undifferentiated human embryonic stem cells. PLoS One 2011;6:e19355.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6439

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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