Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Titration ELISA analysis of LAG-3 sdAbs to detect recombinant LAG-3 (extracellular domain) coated at 1 ug/mL. sdAbs are detected with a mouse mAb against a C-terminal myc-tag followed by a goat anti-mouse IgG-HRP conjugate.
Immunohistochemistry of LAG-3 in human lymph node tissue with LAG-3 single domain antibody at 1 μg/mL.
Flow cytometry analysis of LAG-3 overexpressing HEK293 cells using LAG-3 single domain antibody at 1 μg/ml. Blue: Untransfected HEK293 cells. Yellow: LAG-3 overexpressing HEK293 cells.
Immunofluorescence of LAG-3 in transfected HEK293 cells with LAG-3 single domain antibody at 5 μg/mL.
Titration ELISA analysis of LAG-3 sdAbs to detect recombinant LAG-3 (extracellular domain) coated at 1 ug/mL. sdAbs are detected with a mouse mAb against a C-terminal myc-tag followed by a goat anti-mouse IgG-HRP conjugate.
Recombinant
Trial Size

LAG-3 Single Domain Antibody [1C6]

CATALOG NUMBER: SD8842

Clonality:
Recombinant Monoclonal
Tested Applications:
ELISA, Flow, IF, IHC-P
Host Species:
Llama
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Llama
Species Reactivity:
Human
Immunogen:
LAG3 antibody was raised against a recombinant protein corresponding to amino acids 23-434 of human LAG3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF, IHC-P
Application Note:
LAG-3 antibody can be used for ELISA starting at 0.1 μg/mL. For immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 5 μg/mL. For flow cytometry at 1 μg/ml.
Purification:
LAG3 Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein.  It also contains a myc-tag for detection.
Clonality:
Recombinant Monoclonal
Isotype:
sdAb
physical-state:
Liquid
Buffer:
LAG-3 Antibody is supplied in PBS.
Concentration:
1 mg/mL
Storage Conditions:
LAG3 antibody should be stored in working aliquots at -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
LAG3
Additional Names:
LAG3 Antibody: lymphocyte activating 3, LAG-3, CD223
Protein Accession Number:
NP_002277
Protein Gi Number:
167614500
Ncbi Gene Id Number:
3902
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2). It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4). LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy.
Background Reference 1:
Huard B, Tournier M, Hercend T, et al. Lymphocyte-activation gene 3/major histocompatibility complex class II interaction modulates the antigenic response of CD4+ T lymphocytes. Eur J Immunol 1994; 24:3216–21.
Background Reference 2:
Triebel F. LAG-3: a regulator of T-cell and DC responses and its use in therapeutic vaccination. Trends Immunol 2003; 24:619–22.
Background Reference 3:
Workman CJ, Wang Y, El Kasmi KC, et al. LAG-3 regulates plasmacytoid dendritic cell homeostasis. J Immunol. 2009; 182:1885–91.
Background Reference 4:
Workman CJ, Vignali DA. The CD4-related molecule, LAG-3 (CD223), regulates the expansion of activated T cells. Eur J Immunol 2003; 33:970–9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

SD8842

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369