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Formalin/paraffin human tonsil stained with Lambda light chain antibody (ICO106).
SDS-PAGE Analysis of Purified, BSA-Free Lambda Light Chain Antibody (clone ICO-106). Confirmation of Integrity and Purity of the Antibody.
Formalin/paraffin human tonsil stained with Lambda light chain antibody (ICO106).

Lambda Light Chain Antibody [ICO-106]

CATALOG NUMBER: 33-152

Clonality:
Monoclonal
Tested Applications:
Flow, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Purified human Ig was used as the immunogen for this Lambda light chain antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC, WB
Application Note:
Flow Cytometry: 0.5-1 ug/million cells
IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1, kappa
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
IGL
Ncbi Gene Id Number:
3535
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
This antibody is specific to lambda light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. In general the ratio of Kappa to Lambda is 3:1. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-152

List Size:
100 ug

List Price:

$626.00

Datasheet
Shipping Info

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