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Western blot analysis of extracts of various cell lines, using Lamin A/C antibody (13-106) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4 mM) at 37 for 20 hours or treated by Paclitaxel (100 nM/ml) at 37 for 20 hours. C6 cells were treated by Hydroxyurea (4 mM) at 37 for 20 hours or treated by Paclitaxel (100 nM) at 37 for 20 hours. <br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Western blot analysis of extracts of various cell lines, using Lamin A/C antibody (13-106) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4 mM) at 37 for 20 hours or treated by Paclitaxel (100 nM/ml) at 37 for 20 hours. C6 cells were treated by Hydroxyurea (4 mM) at 37 for 20 hours or treated by Paclitaxel (100 nM) at 37 for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

Lamin A/C Antibody

CATALOG NUMBER: 13-106

Clonality:
Polyclonal
Tested Applications:
IF, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 403-572 of human Lamin C (NP_005563.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:50 - 1:200
Positive Control 1:
HeLa
Positive Control 2:
C6
Predicted Molecular Weight:
Observed: 69kDa, 78kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
LMNA
Additional Names:
LMNA, 70 kDa lamin, CDCD1, CMD1A, EMD2, FPLD, IDC, FPL, FPLD2, Lamin A/C, Lamin, LFP, LMNC, LMN1, LMNL1, HGPS, Prelamin-A/C, PRO1, Lamin A, Lamin A/C-like 1, LGMD1B, CDDC, CMT2B1, LDP1
Ncbi Gene Id Number:
4000
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-106

List Size:
100 uL

List Price:

$436.00

Datasheet
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