Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of extracts of various cell lines, using Lamin A / C antibody (16-912) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3s.
Immunohistochemistry of paraffin-embedded human mammary cancer using Lamin A / C antibody (16-912) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human uterine cancer using Lamin A / C antibody (16-912) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using Lamin A / C antibody (16-912) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using Lamin A / C antibody (16-912) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using Lamin A / C antibody (16-912) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using Lamin A / C antibody (16-912) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using Lamin A / C antibody (16-912) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using Lamin A / C antibody (16-912) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of NIH-3T3 cells using BLOC1S3 antibody (16-904) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using Lamin A / C antibody (16-912) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using Lamin A / C antibody (16-912) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using Lamin A / C antibody (16-912) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.

Lamin A / C Antibody

CATALOG NUMBER: 16-912

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide of human Lamin A / C
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:200 - 1:1000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
HeLa
Positive Control 2:
K562
Positive Control 3:
A-549
Positive Control 4:
NIH/3T3
Positive Control 5:
mouse ovary
Predicted Molecular Weight:
Observed: 74kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
LMNA
Additional Names:
LMNA, 70 kDa lamin, CDCD1, CMD1A, EMD2, FPLD, IDC, FPL, FPLD2, Lamin A/C, Lamin, LFP, LMNC, LMN1, LMNL1, HGPS, Prelamin-A/C, PRO1, Lamin A, Lamin A/C-like 1, LGMD1B, CDDC, CMT2B1, LDP1
Ncbi Gene Id Number:
4000
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-912

List Size:
100 uL

List Price:

$540.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369