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Western blot analysis of extracts of various cell lines, using Lamin B1 antibody (16-793) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human breast using Lamin B1 antibody (16-793) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using Lamin B1 antibody (16-793) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using Lamin B1 antibody (16-793) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using Lamin B1 antibody (16-793) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using Lamin B1 antibody (16-793) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using Lamin B1 antibody (16-793) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

Lamin B1 Antibody

CATALOG NUMBER: 16-793

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide of human Lamin B1
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
Jurkat
Positive Control 2:
HT-29
Positive Control 3:
HeLa
Positive Control 4:
U-251MG
Positive Control 5:
mouse liver
Positive Control 6:
mouse brain
Predicted Molecular Weight:
Observed: 75kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
LMNB1
Additional Names:
LMNB1, lamin B1, ADLD, LMN, LMN2, LMNB, MGC111419, OTTHUMP00000159218, lamin-B1
Ncbi Gene Id Number:
4001
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-793

List Size:
100 uL

List Price:

$540.00

Datasheet
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