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Western blot analysis of Lamin B1 antibody in Ramos cell line lysates (35ug/lane)
Lamin B1 Antibody IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis of Lamin B1 antibody in Ramos cell line lysates (35ug/lane)

Lamin B1 Antibody

CATALOG NUMBER: 62-344

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This Lamin B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 459-488 amino acids from the C-terminal region of human Lamin B1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
66 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
LMNB1
Additional Names:
Lamin-B1, LMNB1, LMN2, LMNB
Protein Accession Number:
P20700
Protein Gi Number:
125953
Ncbi Gene Id Number:
4001
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Lamin B1 is one of the two B type proteins, B1.
Background Reference 1:
Martin,C., J. Cell. Sci. 122 (PT 10), 1551-1562 (2009)
Background Reference 2:
Brussino,A., J. Neurol. Neurosurg. Psychiatr. 80 (2), 237-240 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-344

List Size:
400 ul

List Price:

$495.00

Datasheet
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