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Western blot analysis of LAMP-2 in HepG2 cell lysate with LAMP-2 antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of LAMP-2 in HepG2 cells with LAMP-2 antibody at 10 μg/mL.
Immunohistochemistry of LAMP-2 in mouse liver tissue with LAMP-2 Antibody at 5 μg/mL.
Western blot analysis of LAMP-2 in HepG2 cell lysate with LAMP-2 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

LAMP-2 Antibody

CATALOG NUMBER: 3627

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (75%), Rat: (75%), Bovine: (75%)
Immunogen:
LAMP-2 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human LAMP-2.
The immunogen is located within the last 50 amino acids of LAMP-2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IHC-P, WB
Application Note:
LAMP-2 antibody can be used for the detection of LAMP-2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL and immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Specificity:
LAMP-2 antibody cross reacts with LAMP2B and LAMP2C, but not LAMP2A.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Positive Control 2:
Cat. No. 17-011 - Hep G2 Cell Slide
Purification:
LAMP-2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
LAMP-2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
LAMP-2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Lamp2
Additional Names:
LAMP-2 Antibody: Mac3, LGP-B, CD107b, Lamp-2, Lamp II, Lamp-2a, Lamp-2b, Lamp-2c, Lysosome-associated membrane glycoprotein 2, CD107 antigen-like family member B, LAMP-2
Protein Accession Number:
NP_054701
Protein Gi Number:
31543108
Ncbi Gene Id Number:
16784
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
LAMP-2 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components and is negatively regulated by TOR (Target of rapamycin). LAMP-2, a highly glycosylated protein associated with the lysosome, has recently been shown to be important in autophagy as mice deficient in this protein failed to convert autophagic vacuoles into vacuoles leading to impaired degradation of long-lived proteins. This correlates with the finding that human LAMP-2 deficiency causing Danon's disease is associated with the accumulation of autophagic material in striated myocytes. LAMP-2 exists in multiple isoforms.
Background Reference 1:
Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906.
Background Reference 2:
Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis1993; 14:2501-5.
Background Reference 3:
Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol.2000; 150:1507-13.
Background Reference 4:
Granger BL, Green SA, Gabel CA, et al. Characterization and cloning of the lgp110, a lysosomal glycoprotein from mouse and rat cells. J. Biol. Chem.1990; 265:12036-43.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3627

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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