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Western blot analysis of LAMTOR1 in A431 cell lysate with LAMTOR1 antibody at 1 μg/ml.
Immunohistochemistry of LAMTOR1 in human small intestine tissue with LAMTOR1 antibody at 5 μg/mL.
Immunofluorescence of LAMTOR1 in human small intestine tissue with LAMTOR1 antibody at 20 μg/mL.
Western blot analysis of LAMTOR1 in A431 cell lysate with LAMTOR1 antibody at 1 μg/ml.
Trial Size

LAMTOR1 Antibody

CATALOG NUMBER: 7709

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
Rabbit polyclonal LAMTOR1 antibody was raised against a 17 amino acid peptide near the amino terminus of human LAMTOR1 .
The immunogen is located within the first 50 amino acids of LAMTOR1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
LAMTOR1 antibody can be used for detection of LAMTOR1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
LAMTOR1 antibody is human, mouse and rat specific. LAMTOR1 antibody is predicted to not cross-react with other LAMTOR family proteins.
Positive Control 1:
Cat. No. 1202 - A431 Cell Lysate
Positive Control 2:
Cat. No. 11-801 - Human Small Intestine Tissue Slide
Predicted Molecular Weight:
Predicted: 18 kDa
Observed: 18 kDa
Purification:
LAMTOR1 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
LAMTOR1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
LAMTOR1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
LAMTOR1
Additional Names:
LAMTOR1 Antibody: p18, PDRO, C11orf59, p27RF-Rho, Ragulator1, PP7157, Ragulator complex protein LAMTOR1, Lipid raft adaptor protein p18
Protein Accession Number:
NP_060377
Protein Gi Number:
8923579
Ncbi Gene Id Number:
55004
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
LAMTOR1 is a 161 amino acid membrane protein belonging to the LAMTOR family. It has been identified to interact with and recruit the four Rag GTPases (Rag A-D) to the surface of lysosomes (1). The mTORC1 kinase complex is a critical component in the regulation of cell growth (2). As part of the ragulator complex, LAMTOR1 recruits the Rag GTPases and the mTORC1 complex to lysosomes, a key step in activation of the TOR signaling cascade by amino acids (3). LAMTOR1 may be involved in cholesterol homeostasis regulating LDL uptake and cholesterol release from late endosomes / lysosomes (4).
Background Reference 1:
Sancak Y, Bar-Peled L, Zoncu R, et al. Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is necessary for its activation by amino acids. Cell 2010; 141:290-303.
Background Reference 2:
Kim E, Goraksha-Hicks P, Li L, et al. Regulation of TORC1 by Rag GTPases in nutrient response. Nat. Cell Biol. 2008; 10:935-45.
Background Reference 3:
Wullschleger S, Loewith R, and Hall MN. TOR signaling in growth and metabolism. Cell 2006; 124:471-84.
Background Reference 4:
Soma-Nagae T, Nada S, Kitagawa M, et al. The lysosomal signaling anchor p18/LAMTOR1 controls epidermal development by regulating lysosome-mediated catabolic processes. J. Cell Sci. 2013; 126:3575-84.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7709

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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