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Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

LARS Antibody

CATALOG NUMBER: 55-704

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Immunogen:
This LARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 176-205 amino acids from the N-terminal region of human LARS.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For FACS starting dilution is: 1:10~50
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
134 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
LARS
Additional Names:
Leucine--tRNA ligase, cytoplasmic, Leucyl-tRNA synthetase, LeuRS, LARS, KIAA1352
Protein Accession Number:
Q9P2J5
Protein Gi Number:
48428689
Ncbi Gene Id Number:
51520
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a cytosolic leucine-tRNA synthetase, amember of the class I aminoacyl-tRNA synthetase family. The encodedenzyme catalyzes the ATP-dependent ligation of L-leucine totRNA(Leu). It is found in the cytoplasm as part of amultisynthetase complex and interacts with the arginine tRNAsynthetase through its C-terminal domain. Alternatively splicedtranscript variants of this gene have been found; however, theirfull-length nature is not known.
Background Reference 1:
Pang, Y.L., et al. Biochemistry 48(38):8958-8964(2009)
Background Reference 2:
Seiradake, E., et al. J. Mol. Biol. 390(2):196-207(2009)
Background Reference 3:
Shin, S.H., et al. Exp. Mol. Med. 40(2):229-236(2008)
Background Reference 4:
Maeso, E., et al. Neuromuscul. Disord. 17(5):415-418(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-704

List Size:
400 ul

List Price:

$495.00

Datasheet
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