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Western Blot at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of anti-LARS Antibody in 293 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with LARS antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western Blot at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 ug per lane.

LARS Antibody

CATALOG NUMBER: 62-892

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This LARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1118-1145 amino acids from the C-terminal region of human LARS.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
134 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
LARS
Additional Names:
Leucine--tRNA ligase, cytoplasmic, Leucyl-tRNA synthetase, LeuRS, LARS, KIAA1352
Protein Accession Number:
Q9P2J5
Protein Gi Number:
48428689
Ncbi Gene Id Number:
51520
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
LARS, a cytosolic leucine-tRNA synthetase, a member of the class I aminoacyl-tRNA synthetase family. This enzyme catalyzes the ATP-dependent ligation of L-leucine to tRNA(Leu). It is found in the cytoplasm as part of a multisynthetase complex and interacts with the arginine tRNA synthetase through its C-terminal domain.
Background Reference 1:
Lue,S.W.; Biochemistry 46 (15), 4466-4472 (2007)
Background Reference 2:
Ling,C., J. Biol. Chem. 280 (41), 34755-34763 (2005)
Background Reference 3:
Giles,R.E., Somatic Cell Genet.(5), 667-687 (1980)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-892

List Size:
400 ul

List Price:

$495.00

Datasheet
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