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Western blot analysis of lysed extracts from HeLa and 293 cells using LC3A Antibody.
Immunofluorescence staining of methanol-fixed HeLa cells using LC3A antibody #21402.
Western blot analysis of lysed extracts from HeLa and 293 cells using LC3A Antibody.

LC3A Antibody

CATALOG NUMBER: 79-628

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
LC3A antibody was raised against a peptide sequence around aa.5~10 (R-P-F-K-Q) derived from Human LC3A.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of total LC3A protein.
Predicted Molecular Weight:
18, 16 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
MAP1LC3A
Additional Names:
LC3, LC3A, ATG8E, MAP1ALC3, MAP1BLC3, Microtubule-associated protein 1 light chain 3 alpha, MAP1A/1B light chain 3 A, MAP1A/MAP1B LC3 A, MAP1 light chain 3-like protein 1, Autophagy-related protein LC3 A
Protein Accession Number:
NP_115903.1
Protein Gi Number:
14210522
Ncbi Gene Id Number:
84557
Background:
Probably involved in formation of autophagosomal vacuoles (autophagosomes).
Background Reference 1:
Hammarback JA, et al. Neuron 7 (1): 129-39, 1991. He H, et al. J. Biol Chem 278 (31): 29278-87, 2003.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-628

List Size:
0.1 mg

List Price:

$370.00

Datasheet
Shipping Info

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