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Western blot analysis of extracts of various cell lines, using LC3A/LC3B antibody (16-124) at 1:1000 dilution. Hela cells were treated by Chloroquine (50 μM) for 20 hours. NIH/3T3 cells were treated by Chloroquine (50 μM) for 20 hours.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human breast cancer using LC3A / LC3B antibody (16-124) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using LC3A / LC3B antibody (16-124) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human mammary cancer using LC3A / LC3B antibody (16-124) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using LC3A / LC3B antibody (16-124) at dilution of 1:100. Hela cells were treated by Chloroquine (50 μM) for 20 hours(left). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using LC3A/LC3B antibody (16-124) at 1:1000 dilution. Hela cells were treated by Chloroquine (50 μM) for 20 hours. NIH/3T3 cells were treated by Chloroquine (50 μM) for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.

LC3A / LC3B Antibody

CATALOG NUMBER: 16-124

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-125 of human LC3A / LC3B (NP_073729.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
HeLa
Positive Control 2:
NIH/3T3
Predicted Molecular Weight:
Observed: 14kDa, 16kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
MAP1LC3A/MAP1LC3B
Ncbi Gene Id Number:
84557;81631
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-124

List Size:
50 uL

List Price:

$309.00

Out of stock

Datasheet
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