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Western blot analysis of extracts of various cell lines, using LC3B antibody (22-775) at 1:1000 dilution. C6 cells were treated by Chloroquine (50 ?M) for 20 hours. Hela cells were treated by Chloroquine (50 ?M) for 20 hours. NIH/3T3 cells were treated by Chloroquine (50 ?M) for 20 hours.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Western blot analysis of extracts of pig liver, using LC3B antibody (22-775).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts from normal (control) and LC3B knockout (KO) 293T cells, using LC3B antibody (22-775) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat heart using LC3B antibody (22-775) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using LC3B antibody (22-775) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using LC3B antibody (22-775) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using LC3B antibody (22-775) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using LC3B antibody (22-775) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using LC3B antibody (22-775) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using HIRIP3 antibody (22-631) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using LC3B antibody (22-775) at dilution of 1:100. Hela cells were treated by Chloroquine (50 ?M) for 20 hours(left). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using LC3B antibody (22-775) at dilution of 1:100. NIH/3T3 cells were treated by Chloroquine (50 ?M) for 20 hours(left). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using LC3B antibody (22-775) at 1:1000 dilution. C6 cells were treated by Chloroquine (50 ?M) for 20 hours. Hela cells were treated by Chloroquine (50 ?M) for 20 hours. NIH/3T3 cells were treated by Chloroquine (50 ?M) for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
KO

LC3B Antibody, KO Validated

CATALOG NUMBER: 22-775

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Pig, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Pig, Rat
Immunogen:
A synthetic peptide of human LC3B
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
C6
Positive Control 2:
HeLa
Positive Control 3:
NIH/3T3
Positive Control 4:
Pig liver
Predicted Molecular Weight:
Observed: 14kDa, 16kDa
Validation:
Antibody is Knockout validated.
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
MAP1LC3B
Additional Names:
MAP1LC3B Antibody: LC3B, ATG8F, MAP1LC3B-a, MAP1A/1BLC3, MAP1ALC3, Autophagy-related protein LC3 B
Ncbi Gene Id Number:
81631
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-775

List Size:
100 uL

List Price:

$540.00

Datasheet
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