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42-990 (0.1ug/ml) staining of Human Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-990 (0.3ug/ml) staining of Rat Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-990 (1ug/ml) staining of Pig Bone Marrow (A) and Spleen (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-990 (0.1ug/ml) staining of Human Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

LCK Antibody

CATALOG NUMBER: 42-990

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Pig, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Pig, Rat
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-RNGSEVRDPLVTYE
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 60kDa band observed in Human and Rat Thymus lysates (calculated MW of 58.0kDa according to NP_005347.3). Recommended concentration: 0.1-0.3ug/ml.Approx 60kDa band observed in Pig Bone Marrow and Spleen lysates (calculated MW of 58.2kDa according to NP_001137185.1). Recommended concentration: 1-3ug/ml.
Specificity:
This antibody is not expected to cross-react with other tyrosine protein kinases. Reported variants represent identical protein: NP_005347.3, NP_001036236.1
Positive Control 1:
Cat. No. 1314 - Human Thymus Tissue Lysate
Predicted Molecular Weight:
Approx 60 kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
LCK
Additional Names:
LCK, lymphocyte-specific protein tyrosine kinase, LSK, YT16, p56lck, pp58lck, OTTHUMP00000008640, OTTHUMP00000008740, OTTHUMP00000008741, T-lymphocyte specific protein tyrosine kinase p56lck, leukocyte C-terminal Src kinase, lymphocyte cell-specific protein-tyrosine kinase, p56(LSTRA) protein-tyrosine kinase, proto-oncogene tyrosine-protein kinase LCK, t cell-specific protein-tyrosine kinase, tyrosine-protein kinase Lck
Protein Accession Number:
NP_005347.3
Protein Gi Number:
112789546
Ncbi Gene Id Number:
3932

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-990

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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