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Western blot analysis of lysates from mouse thymus and rat thymus tissue lysate(from left to right), using LSK Antibody (I37) at 1:1000 at each lane.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Confocal immunofluorescent analysis of LCK Antibody with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of lysates from mouse thymus and rat thymus tissue lysate(from left to right), using LSK Antibody (I37) at 1:1000 at each lane.

LCK Antibody

CATALOG NUMBER: 63-164

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This LCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-52 amino acids from the N-terminal region of human LCK.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
58 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
LCK
Additional Names:
Tyrosine-protein kinase Lck, Leukocyte C-terminal Src kinase, LSK, Lymphocyte cell-specific protein-tyrosine kinase, Protein YT16, Proto-oncogene Lck, T cell-specific protein-tyrosine kinase, p56-LCK, LCK
Protein Accession Number:
P06239
Protein Gi Number:
125474
Ncbi Gene Id Number:
3932
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
LSK, which belongs to the SRC subfamily of Tyr protein kinases, may participate in antigen-induced T-cell activation. It binds to phosphatidylinositol 3'-kinase (PI3K) from T lymphocytes through its SH3 domain and to the tyrosine phosphorylated form of KHDRBS1/p70 through its SH2 domain. LSK is bound to the cytoplasmic domain of either CD4 or CD8. This protein is involved in leukemias by a chromosomal translocation t(1;7)(p34;q34) which involves LCK and T-cell receptor beta chain (TCRB) genes.
Background Reference 1:
Harris, R.A., et al., Proteomics 2(2):212-223 (2002). Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002). Boncristiano, M., et al., Eur. J. Immunol. 30(9):2632-2638 (2000). Tong, L., et al., J. Biol. Chem. 273(32):20238-20242 (1998). Yamaguchi, H., et al., Nature 384(6608):484-489 (1996).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-164

List Size:
400 ul

List Price:

$495.00

Datasheet
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