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Western blot analysis using LCK monoclonal antibody against truncated LCK recombinant protein and Jurkat cell lysate.
Western blot analysis using LCK monoclonal antibody against truncated LCK recombinant protein and Jurkat cell lysate.

Lck Antibody [8E5F9 / 4B2C7]

CATALOG NUMBER: 32-186

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Ni-NTA purified truncated recombinant Lck expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Western Blot:1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Clonality:
Monoclonal
Isotype:
IgG2a
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
Lck monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
LCK
Additional Names:
Leukocyte C-terminal Src kinase, LSK, YT16, p56lck, pp58lck
Protein Accession Number:
P06239
Protein Gi Number:
125474
Ncbi Gene Id Number:
3932
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Lck(lymphocyte-specific protein tyrosine kinase), with 509-amino acid protein (about 56 kDa), belongs to the Src non-receptor tyrosine kinases family. By virtue of common structural motifs, the Src family is composed of nine members in vertebrates, including Src, Yes, Fgr, Frk, Fyn, Lyn, Hck, Lck and Blk. Lck is expressed predominantly in T cells and is localized to the inner surface of the plasma membrane. Lck is activated after T cell stimulation and is required for T-cell proliferation and IL-2 production. Aberrant expression or activation of Lck kinase has been reported in both lymphoid and nonlymphoid malignancies. In addition, inhibition of Lck has been a target to prevent lymphocyte activation and acute rejection.
Background Reference 1:
Mingjian Shi, John C. Cooper, Chao-Lan Yu. Mol. Cancer Res., Jan 2006; 4: 39–45
Background Reference 2:
Robert F. Stachlewitz, Michelle A. Hart, Brian Bettencourt. J. Pharmacol. Exp. Ther., Oct 2005; 315: 36–41.
Background Reference 3:
Ibrahim Y. Hawash, Kamala P. Kesavan, Anthony I. Magee. J. Biol. Chem., Feb 2002; 277: 5683–5691.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-186

List Size:
0.1 mL

List Price:

$495.00

Datasheet
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