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45-081 (0.3ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-081 (0.3ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

LCP2 Antibody

CATALOG NUMBER: 45-081

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: ALRNVPFRSEV-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 80kDa band observed in lysates of cell line Jurkat (calculated MW of 60.2kDa according to NP_005556.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Jackman et al, J Biol Chem. 1995 Mar 31;270(13):7029-32. PMID: 7706237). Recommended concentration: 0.3-1ug/ml.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
LCP2
Additional Names:
LCP2, SLP76, SLP-76, lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa), 76 kDa tyrosine phosphoprotein, SH2 domain-containing leukocyte protein of 76kD, lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kD), lymphocyte cytosolic protein 2
Protein Accession Number:
NP_005556.1
Protein Gi Number:
5031855
Ncbi Gene Id Number:
3937
Background Reference 1:
Jackman JK, Motto DG, Sun Q, Tanemoto M, Turck CW, Peltz GA, Koretzky GA, Findell PR. Molecular cloning of SLP-76, a 76- kDa tyrosine phosphoprotein associated with Grb2 in T cells. J Biol Chem. 1995 Mar 31;270(13):7029-32.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-081

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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