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43-515 (0.3ug/ml) staining of Heart (A), Skeletal Muscle (B) and Kidney (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-515 (0.3ug/ml) staining of Heart (A), Skeletal Muscle (B) and Kidney (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

LDHA Antibody

CATALOG NUMBER: 43-515

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-SDLVKVTLTSE
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 35kDa band observed in Human Heart, Skeletal Muscle and Kidney lysates (calculated MW of 36.7kDa according to NP_005557.1). Recommended concentration: 0.3-1ug/ml.
Specificity:
This antibody is expected to recognize isoform 1, 2 and 3 (NP_005557.1; NP_001128711.1; NP_001158886.1).
Predicted Molecular Weight:
Approx 35kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
LDHA
Additional Names:
LDHA, lactate dehydrogenase A, GSD11, LDH1, LDHM, L-lactate dehydrogenase A chain, LDH muscle subunit, LDH-A, LDH-M, cell proliferation-inducing gene 19 protein, lactate dehydrogenase M, proliferation-inducing gene 19, renal carcinoma antigen NY-REN-59
Protein Accession Number:
NP_005557.1,NP_001128711.1,NP_001158886.1
Protein Gi Number:
5031857
Ncbi Gene Id Number:
3939
Background Reference 1:
Zhang Y, Zhang X, Wang X, Gan L, Yu G, Chen Y, Liu K, Li P, Pan J, Wang J, Qin S.Inhibition of LDH-A by lentivirus-mediated small interfering RNA suppresses intestinal-type gastric cancer tumorigenicity through the downregulation of Oct4.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-515

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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