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Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
Confocal immunofluorescent analysis of LDHA Antibody with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
LDHA Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).

LDHA Antibody

CATALOG NUMBER: 57-107

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This LDHA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 204-232 amino acids from the C-terminal region of human LDHA.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
37 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
LDHA
Additional Names:
L-lactate dehydrogenase A chain, LDH-A, Cell proliferation-inducing gene 19 protein, LDH muscle subunit, LDH-M, Renal carcinoma antigen NY-REN-59, LDHA
Protein Accession Number:
P00338
Protein Gi Number:
126047
Ncbi Gene Id Number:
3939
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene catalyzes the conversionof L-lactate and NAD to pyruvate and NADH in the final step ofanaerobic glycolysis. The protein is found predominantly in muscletissue and belongs to the lactate dehydrogenase family. Mutationsin this gene have been linked to exertional myoglobinuria. Multipletranscript variants encoding different isoforms have been found forthis gene. The human genome contains several non-transcribedpseudogenes of this gene.
Background Reference 1:
Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Background Reference 2:
Zhu, X., et al. Genet. Epidemiol. 34(2):171-187(2010)
Background Reference 3:
Zhuang, L., et al. Mod. Pathol. 23(1):45-53(2010)
Background Reference 4:
Zhao, Y.H., et al. Oncogene 28(42):3689-3701(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-107

List Size:
400 ul

List Price:

$495.00

Datasheet
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