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Western blot analysis in Ramos,A375 cell line lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human skin tissue reacted with LDHA Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in Ramos,A375 cell line lysates (35ug/lane).

LDHA Antibody

CATALOG NUMBER: 63-987

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Monkey, Pig, Rat
Immunogen:
This LDHA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-182 amino acids from the Central region of human LDHA.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
37 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
LDHA
Additional Names:
L-lactate dehydrogenase A chain, LDH-A, Cell proliferation-inducing gene 19 protein, LDH muscle subunit, LDH-M, Renal carcinoma antigen NY-REN-59, LDHA
Protein Accession Number:
P00338
Protein Gi Number:
126047
Ncbi Gene Id Number:
3939
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
LDHA catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria.
Background Reference 1:
Zhu, X., et al. Genet. Epidemiol. 34(2):171-187(2010)
Background Reference 2:
Zhuang, L., et al. Mod. Pathol. 23(1):45-53(2010)
Background Reference 3:
Zhao, Y.H., et al. Oncogene 28(42):3689-3701(2009)
Background Reference 4:
Xie, H., et al. Mol. Cancer Ther. 8(3):626-635(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-987

List Size:
400 ul

List Price:

$495.00

Datasheet
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