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Western blot analysis of LDL-R in human liver tissue lysate with LDL-R antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of LDL-R in human liver tissue with LDL-R antibody at 2.5 μg/mL.
Immunofluorescence of LDLR in human liver tissue with LDLR antibody at 20 μg/mL.
Western blot analysis of LDL-R in human liver tissue lysate with LDL-R antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

LDL-R Antibody

CATALOG NUMBER: 5163

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Chicken
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Chicken
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (89%), Rabbit: (80%)
Immunogen:
LDL-R antibody was raised against an 18 amino acid synthetic peptide near the center of human LDL-R.
The immunogen is located within amino acids 490 - 540 of LDL-R.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
LDL-R antibody can be used for detection of LDL-R by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1304 - Human Liver Tissue Lysate
Positive Control 2:
Cat. No. 10-201 - Human Liver Tissue Slide
Purification:
LDL-R Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgY
physical-state:
Liquid
Buffer:
LDL-R Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
LDL-R antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
LDLR
Additional Names:
LDL-R Antibody: FH, FHC, LDLCQ2, Low-density lipoprotein receptor, LDL receptor
Protein Accession Number:
NP_000518
Protein Gi Number:
4504975
Ncbi Gene Id Number:
3949
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
LDL-R Antibody: The low density lipoprotein receptor (LDL-R) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in the LDL-R gene cause the autosomal dominant disorder, familial hypercholesterolemia. Along with SCARB1, CLDN1, and the tetraspanin superfamily member CD81, LDL-R has been reported to be an entry factor for the Hepatitis C virus. At least three isoforms of LDL-R are known to exist.
Background Reference 1:
Li Y, Cam J, and Bu G. Low-density lipoprotein receptor family: endocytosis and signal transduction. Mol. Neurobiol.2001; 23:53-67.
Background Reference 2:
Austin MA, Hutter CM, Zimmern RL, et al. Familial hypercholesterolemia and coronary heart disease: a HuGE association review. Am. J. Epidemiol.2004; 160:421-9.
Background Reference 3:
Helle F and Dubuisson J. Hepatitis C virus entry into host cells. Cell Mol. Life Sci.2008; 65:100-12.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5163

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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