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Western blot analysis of extracts of mouse liver, using LDLRAP1 antibody (22-691) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using LDLRAP1 antibody (22-691) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using LDLRAP1 antibody (22-691) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A-549 cells using LDLRAP1 antibody (22-691).
Western blot analysis of extracts of mouse liver, using LDLRAP1 antibody (22-691) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

LDLRAP1 Antibody

CATALOG NUMBER: 22-691

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 59-308 of human LDLRAP1 (NP_056442.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:100
Positive Control 1:
Mouse liver
Predicted Molecular Weight:
Observed: 38kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
LDLRAP1
Additional Names:
ARH, ARH2, FHCB1, FHCB2, MGC34705, DKFZp586D0624, LDL receptor adaptor protein, low density lipoprotein receptor adaptor protein 1, ARH
Ncbi Gene Id Number:
26119
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a cytosolic protein which contains a phosphotyrosine binding (PTD) domain. The PTD domain has been found to interact with the cytoplasmic tail of the LDL receptor. Mutations in this gene lead to LDL receptor malfunction and cause the disorder autosomal recessive hypercholesterolaemia.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-691

List Size:
100 uL

List Price:

$436.00

Datasheet
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