LEC Antibody [1.2_5G3-1D7]

CATALOG NUMBER: XP-5722-M

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
The monoclonal antibody was produced in BALB/c X ICR F1 mice using recombinant hLEC as the immunizing antigen.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ELISA:
In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 500 pg/mL of recombinant human LEC when used with our biotinylated antigen affinity purified anti-human LEC (XP-5722-M) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.
Western Blot:
To detect hLEC by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLEC is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.
Purification:
This IgG1K antibody was purified from ascites fluid on a Protein A affinity column.
Clonality:
Monoclonal
Physical State:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
LEC lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.
Ncbi Official Symbol:
CCL16
Additional Names:
CCL16, CKb12, HCC4, ILINCK, LCC1, Liver expressed chemokine, LMC, Monotactin 1, Mtn1, NCC4, SCYA16, SCYL4, Small inducible cytokine A16 precursor, Small inducible cytokine subfamily A (Cys Cys) member 16, C-C motif chemokine 16, Chemokine CC-4, HCC-4, LEC, LCC-1, Mtn-1, NCC-4
Protein Accession Number:
O15467
Protein Gi Number:
6094382
Ncbi Gene Id Number:
6360
User Note:
Centrifuge vial prior to opening.
Background:
LEC displays chemotactic activity for lymphocytes and monocytes but not for neutrophils. It also shows a potent myelosuppressive activity and suppresses proliferation of myeloid progenitor cells. Its expression is upregulated by IL10.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5722-M

List Size:
0.5 mg

List Price:

$376.00

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