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Western blot analysis of anti-LEFTB Pab in HL60 cell line lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with LEFTB antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of anti-LEFTB Pab in HL60 cell line lysates (35ug/lane).

Lefty Antibody

CATALOG NUMBER: 62-803

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This Lefty (LEFTB) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 283-314 amino acids from the C-terminal region of human Lefty (LEFTB).
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
41 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
LEFTY1
Additional Names:
Left-right determination factor 1, Left-right determination factor B, Protein lefty-1, Protein lefty-B, LEFTY1, LEFTB, LEFTYB
Protein Accession Number:
O75610
Ncbi Gene Id Number:
10637
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
LEFTB is a member of the TGF-beta family of proteins. A similar secreted protein in mouse plays a role in left-right asymmetry determination of organ systems during development. Alternative processing of this protein can yield three different products.
Background Reference 1:
Kosaki K.,Am. J. Hum. Genet. 64:712-721(1999)
Background Reference 2:
Dvash,T.,Stem Cells 25 (2), 465-472 (2007)
Background Reference 3:
Besser,D.,J. Biol. Chem. 279 (43), 45076-45084 (2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-803

List Size:
400 ul

List Price:

$495.00

Datasheet
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