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Antibody used in WB on Human RPMI 8226 at 0.2-1 ug/ml.
Antibody used in IHC on Human Uterus at 5 ug/ml.
Antibody used in WB on Human RPMI 8226 at 0.2-1 ug/ml.

LEFTY2 Antibody

CATALOG NUMBER: 29-698

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human LEFTY2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
LEFTY2 antibody can be used for detection of LEFTY2 by ELISA at 1:312500. LEFTY2 antibody can be used for detection of LEFTY2 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
RPMI 8826 Cell Lysate
Predicted Molecular Weight:
40 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store LEFTY2 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
LEFTY2
Additional Names:
LEFTY2, EBAF, LEFTA, LEFTYA, MGC46222, TGFB4
Protein Accession Number:
NP_003231
Protein Gi Number:
27436881
Ncbi Gene Id Number:
7044
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
LEFTY2 is a member of the TGF-beta family of proteins. The protein is secreted and plays a role in left-right asymmetry determination of organ systems during development. The protein may also play a role in endometrial bleeding. Mutations in its gene have been associated with left-right axis malformations, particularly in the heart and lungs. Some types of infertility have been associated with dysregulated expression of its gene in the endometrium.This gene encodes a member of the TGF-beta family of proteins. The encoded protein is secreted and plays a role in left-right asymmetry determination of organ systems during development. The protein may also play a role in endometrial bleeding. Mutations in this gene have been associated with left-right axis malformations, particularly in the heart and lungs. Some types of infertility have been associated with dysregulated expression of this gene in the endometrium. Alternative processing of this protein can yield three different products. This gene is closely linked to both a related family member and a related pseudogene.
Background Reference 1:
Cornet, P.B., (2005) J. Clin. Endocrinol. Metab. 90 (2), 1001-1011.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

29-698

List Size:
100 ul

List Price:

$519.00

Datasheet
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