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Western blot analysis of LEMD3 in human colon tissue lysate with LEMD3 antibody at 1 μg/mL.
Immunohistochemistry of LEMD3 in human colon tissue with LEMD3 antibody at 2.5 μg/mL.
Immunofluorescence of LEMD3 in human colon tissue with LEMD3 antibody at 5 μg/mL.
Western blot analysis of LEMD3 in human colon tissue lysate with LEMD3 antibody at 1 μg/mL.
Trial Size

LEMD3 Antibody

CATALOG NUMBER: 6603

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
LEMD3 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human LEMD3.
The immunogen is located within the last 50 amino acids of LEMD3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
LEMD3 antibody can be used for detection of LEMD3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least three isoforms of LEMD3 are known to exist; this antibody will detect all isoforms. LEMD3 antibody is predicted to not cross-react with LEMD1 and LEMD2
Positive Control 1:
Cat. No. 1320 - Human Colon Tissue Lysate
Purification:
LEMD3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
LEMD3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
LEMD3 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
LEMD3
Additional Names:
LEMD3 Antibody: MAN1, MAN1, Inner nuclear membrane protein Man1, LEM domain-containing protein 3
Protein Accession Number:
NP_055134
Protein Gi Number:
7706607
Ncbi Gene Id Number:
23592
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
LEMD3 Antibody: LEMD3 (LEM domain-containing 3) is a lem-domain containing protein, also known as MAN1, whose loss of function results in disorders characterized by osteopoikilosis, Buschke-Ollendorff syndrome, and melorheostosis. LEMD3 is an inner nuclear membrane protein that interacts with BMP and activin-TGF-beta receptor-activated Smads and antagonizes TGF-beta signaling in human cells.
Background Reference 1:
Hellemans J, Preobrazhenska O, Willaert A, et al. Loss-of-function mutations in LEMD3 result in osteopoikilosis, Buschke-Ollendorff syndrome, and melorheostosis. Nat. Genet. 2004; 36:1213-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6603

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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