Leptin Antibody

CATALOG NUMBER: 36-200

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Rat
Immunogen:
Recombinant rat leptin.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ELISA: (direct and indirect: 1:5,000-1:10,000). Western Blot: (1:5,000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
Specificity:
Recognizes rat leptin. Weakly cross-reacts with human and mouse leptin. Detects a band of ~16kDa by Western blot.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
Concentration:
1 mg/ml
Storage Conditions:
Stable for at least 6 months after receipt when stored at -20°C.
Ncbi Official Symbol:
Lep
Additional Names:
OB Gene Product; Obese Factor
Protein Accession Number:
1709436
Protein Gi Number:
P50596
Ncbi Gene Id Number:
25608
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Leptin is involved in the signaling pathway that regulates the size of the body fat depot. An increase in the level of leptin may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. Defects in the leptin gene may be a cause of autosomal recessive obesity.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

36-200

List Size:
100 ug

List Price:

$423.00

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