Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

To detect mLeptin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Murine Leptin (XP-5211Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mLeptin.
To detect mLeptin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mLeptin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect mLeptin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mLeptin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained 4% PFA, paraffin-embedded sections of Trichuris muris infected murine cecum and large intestine tissue. The primary antibody was incubated at 0.20 ug/ml overnight at 4°C and the secondary antibody was a cyanine-3 conjugated Donkey anti-Rabbit (Jackson ImmunoResearch). Heat induced antigen retrieval with a 100mM citric acid was used. Information and photo are courtesy of David Artis, University of Pennsylvania. Optimal concentrations and conditions may vary.
This antibody stained 4% PFA, paraffin-embedded sections of Trichuris muris infected murine cecum and large intestine tissue. The primary antibody was incubated at 0.20 ug/ml overnight at 4°C and the secondary antibody was a cyanine-3 conjugated Donkey anti-Rabbit (Jackson ImmunoResearch). Heat induced antigen retrieval with a 100mM citric acid was used. Information and photo are courtesy of David Artis, University of Pennsylvania. Optimal concentrations and conditions may vary.
To detect mLeptin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Murine Leptin (XP-5211Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mLeptin.

Leptin Antibody

CATALOG NUMBER: XP-5211

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant murine Leptin.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ELISA:
To detect Leptin by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant Leptin.
Sandwich:
To detect mLeptin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine Leptin (XP-5211Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mLeptin.
Western Blot:
To detect Leptin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Leptin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Purification:
Anti-murine Leptin specific antibody was purified by affinity chromatography employing immobilized murine Leptin matrix.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
Leptin antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
Lep
Additional Names:
ob, obese, Ob, Leptin, Obesity factor,
Protein Accession Number:
P41160
Protein Gi Number:
730219
Ncbi Gene Id Number:
16846
User Note:
Centrifuge vial prior to opening.
Background:
Leptin plays a critical role in the regulation of body weight by inhibiting food intake and stimulating energy expenditure. Defects in Leptin production cause severe hereditary obesity in rodents and humans. In addition to its effects on body weight, leptin has a variety of other functions, including the regulation of hematopoiesis, angiogenesis, wound healing, and the immune and inflammatory response. The Leptin gene is the human homolog of the gene (ob) mutant in the mouse 'obese' phenotype.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5211

List Size:
0.1 mg

List Price:

$408.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369