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Leptin Antibody [G1_4B6.3]

CATALOG NUMBER: 38-235

Clonality:
Monoclonal
Tested Applications:
ELISA, Neutralization Assay, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Leptin antibody is produced in BALB/c mice using highly pure (>98%) recombinant human Leptin as the immunizing antigen.
Conjugate:
Unconjugated
Tested Applications:
ELISA, Neutralization Assay, WB
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mIL-2 (1.0 ng/mL), a concentration of 10.0-14.0 μg/mL of this antibody is required. ELISA:
In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 32 pg/mL of recombinant murine IL-2 when used with our biotinylated antigen affinity purified anti-murine IL-2 (XP-5183Bt) as the detection antibody at a concentration of at least 1.0-2.0 μg/mL.
Western Blot:
To detect mIL-2 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-2 is 1.0-2.0 ng/lane, under reducing conditions and 0.2-0.4 ng/lane, under non-reducing conditions.
Purification:
This IgG1K antibody was purified from cell culture by Protein A affinity chromatography.
Clonality:
Monoclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
Leptin lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.
Ncbi Official Symbol:
LEP
Additional Names:
OB, OBS, Leptin, Obese proteinOB, LEPD
Protein Accession Number:
P41159
Protein Gi Number:
730218
Ncbi Gene Id Number:
3952
User Note:
Centrifuge vial prior to opening.
Background:
Leptin plays a critical role in the regulation of body weight by inhibiting food intake and stimulating energy expenditure. Defects in Leptin production cause severe hereditary obesity in rodents and humans. In addition to its effects on body weight, leptin has a variety of other functions, including the regulation of hematopoiesis, angiogenesis, wound healing, and the immune and inflammatory response. The Leptin gene is the human homolog of the gene (ob) mutant in the mouse 'obese' phenotype.

Was product number XP-5712-M

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

38-235

List Size:
0.5 mg

List Price:

$376.00

Datasheet
Shipping Info

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