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Western blot analysis in HL-60,K562,Hela cell line lysates (35ug/lane).
LFNG Antibody IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in HL-60,K562,Hela cell line lysates (35ug/lane).

LFNG Antibody

CATALOG NUMBER: 63-988

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This LFNG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-114 amino acids from the Central region of human LFNG.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
42 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
LFNG
Additional Names:
Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe, O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase, LFNG
Protein Accession Number:
Q8NES3
Protein Gi Number:
27734417
Ncbi Gene Id Number:
3955
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
LFNG belongs to evolutionarily conserved glycosyltransferases that act in the Notch signaling pathway to define boundaries during embryonic development. While their genomic structure is distinct from other glycosyltransferases, fringe proteins have a fucose-specific beta-1,3-N-acetylglucosaminyltransferase activity that leads to elongation of O-linked fucose residues on Notch, which alters Notch signaling. This protein is predicted to be a single-pass type II Golgi membrane protein but it may also be secreted and proteolytically processed like the related proteins in mouse and Drosophila (PMID: 9187150).
Background Reference 1:
Dunwoodie, S.L. Biochim. Biophys. Acta 1792(2):100-111(2009)
Background Reference 2:
Reedijk, M., et al. Int. J. Oncol. 33(6):1223-1229(2008)
Background Reference 3:
Sparrow, D.B., et al. Am. J. Hum. Genet. 78(1):28-37(2006)
Background Reference 4:
Cole, S.E., et al. Dev. Cell 3(1):75-84(2002)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-988

List Size:
400 ul

List Price:

$495.00

Datasheet
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