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Western blot analysis in MCF-7 cell line lysates (35ug/lane).
Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in MCF-7 cell line lysates (35ug/lane).

LGALS3 Antibody

CATALOG NUMBER: 56-128

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This LGALS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 163-191 amino acids from the C-terminal region of human LGALS3.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
26 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
LGALS3
Additional Names:
Galectin-3, Gal-3, 35 kDa lectin, Carbohydrate-binding protein 35, CBP 35, Galactose-specific lectin 3, Galactoside-binding protein, GALBP, IgE-binding protein, L-31, Laminin-binding protein, Lectin L-29, Mac-2 antigen, LGALS3, MAC2
Protein Accession Number:
P17931
Protein Gi Number:
215274262
Ncbi Gene Id Number:
3958
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the galectin family ofcarbohydrate binding proteins. Members of this protein family havean affinity for beta-galactosides. The encoded protein ischaracterized by an N-terminal proline-rich tandem repeat domainand a single C-terminal carbohydrate recognition domain. Thisprotein can self-associate through the N-terminal domain allowingit to bind to multivalent saccharide ligands. This proteinlocalizes to the extracellular matrix, the cytoplasm and thenucleus. This protein plays a role in numerous cellular functionsincluding apoptosis, innate immunity, cell adhesion and T-cellregulation. Alternate splicing results in multiple transcriptvariants.
Background Reference 1:
Salomonsson, E., et al. J. Biol. Chem. 285(45):35079-35091(2010)
Background Reference 2:
Debierre-Grockiego, F., et al. J. Biol. Chem. 285(43):32744-32750(2010)
Background Reference 3:
Zhou, J.Y., et al. J. Proteome Res. 9(10):5133-5141(2010)
Background Reference 4:
Markowska, A.I., et al. J. Exp. Med. 207(9):1981-1993(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-128

List Size:
400 ul

List Price:

$495.00

Datasheet
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